应用实例

植物逆境胁迫&基因表达调控

Analysis of CtHsfA2bbinding to ProAtApx2 in vivo with using luciferase(LUC) assay in Arabidopsisprotoplast.Signs of +/- indicates with/without the components listed on theleft side.
Wang, X. et al. Transcriptional regulation ofheat shock proteins and ascorbate peroxidase by CtHsfA2b from Africanbermudagrass conferring heat tolerance in Arabidopsis.Sci. Rep.6,28021;doi:10.1038/srep28021(2016)

植物克隆筛选

Arabidopsis thaliana seedlings transfected with luciferase (right) and non-transfected (left), 3min exposure after 1 mM luciferin was sprayed onto the leaves.

植物微生物侵染研究

BIK1 and FLS2 interact with RbohD in N. benthamiana. The indicated constructs were transiently expressed in N. benthamiana, and luciferase complementation imaging assay was performed.

转基因植物鉴定

GFP expression, GFP-transfected (right) and Control (left) grain of rice and leaves, Epi excitation 470nm and emission filter f565, exposure time 2sec.